WebIn eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. WebPhospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb detects endogenous levels of H2A.X only when phosphorylated at Ser139. Species Reactivity: Human, Mouse, Rat, Monkey …
(9718) Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb
WebPVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human Phospho-Histone H2AX (S139) Monoclonal Antibody (Catalog # MAB2288) followed by HRP-conjugated … WebWestern blot analysis of 293T, using Phospho-HistoneH2AX-S139 antibody (AP0099) at 1:434 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. slytherin gift ideas
Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone …
WebHowever, similar levels of total Histone H2A.X protein from both untreated and treated lysates are shown by western analysis. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 is shown in the bottom figure. Show Less Show More WebDetection of Human Phospho-Histone H2AX (S139) in Camptothecin-treated PBMC by Flow Cytometry. Human PBMC untreated (open histogram) or treated with 1 µM Camptothecin (Catalog # 1100 , filled histogram) overnight were stained with Rabbit Anti-Human Phospho-Histone H2AX (S139) Monoclonal Antibody (Catalog # MAB2288), followed by PE … WebHistone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as γH2AX when phosphorylated at Ser139. slytherin gift set