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WebApplied Biosystems™ SYBR™ Green PCR Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time. • SYBR™ Green I dye detects double-stranded … Web12 de jan. de 2024 · mir--p在制备诊断或治疗心肌损伤的试剂或药物中的应用技术领域.本发明属于生物药物技术领域,具体涉及mir--p在制备诊断或治疗心肌损伤的试剂或药物中的应用。背景技术.心脏局组织内血管紧张素ii(angii)已被证明在各种心脏疾病的病理过程中发挥重要的促进作用。angii可通过产生过量的活性氧 ... philippines army vehicles
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Web7 de fev. de 2024 · This process is called reverse-transcription polymerase chain reaction. The resulting readings provide information about the amount of otherwise difficult-to … WebTechnology Overview: SYBR Green qPCR. In quantitative PCR, DNA amplification is monitored at each cycle of PCR. When the DNA is in the log linear phase of amplification, the amount of fluorescence increases above the background. The point at which the fluorescence becomes measurable is called the Quantification Cycle (C q) or crossing point. WebPrincípios do PCR. O PCR permite a amplificação de um segmento específico de DNA a partir de uma mistura complexa de DNA contendo uma pequena quantidade do material de interesse [1].O protocolo exige a presença da amostra a ser analisada, primers, dNTPs e DNA polimerase [1].Geralmente, a amostra a ser analisada consiste no DNA … philippines army uniform