Dna helicase pif1
WebUsing recombinant proteins, we found that acetylation stimulated the DNA binding affinity, ATPase activity, and DNA unwinding activities of Pif1. All … WebMar 29, 2024 · PIF1 has a role in DNA resection, specifically for defined DNA regions, such as those prone to form G-quadruplexes. The functions of human PIF1 (hPIF1) are also …
Dna helicase pif1
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WebFeb 20, 2024 · PIF1 family helicases are conserved from bacteria to man. With the exception of the well-studied yeast PIF1 helicases (e.g., ScPif1 and ScRrm3), however, very little is known about how these enzymes help maintain genome stability. WebNov 16, 2024 · DOI: 10.1074/jbc.RA118.004499 Abstract In addition to unwinding double-stranded nucleic acids, helicase activity can also unfold noncanonical structures such as G-quadruplexes. We previously characterized Pif1 helicase catalyzed unfolding of parallel G-quadruplex DNA.
WebApr 18, 2024 · We showed that the Pif1 and Rrm3 DNA helicases promote efficient fork convergence and completion of DNA synthesis, even in the absence of type II topoisomerase. Furthermore, Rrm3 and Pif1 are also important for termination of plasmid DNA replication in vivo. WebUsing recombinant proteins, we found that acetylation stimulated the DNA binding affinity, ATPase activity, and DNA unwinding activities of Pif1. All …
WebSep 11, 2013 · The helicase activity of Pif1 is required for the stimulation of Polδ-mediated DNA synthesis, as revealed by analysing the pif1 (K264A) mutant 13, 20 ( Fig. 3a ). … WebMay 28, 2024 · Pif1 family helicases represent a highly conserved class of enzymes involved in multiple aspects of genome maintenance. Many Pif1 helicases are multi …
WebOct 15, 2024 · Pif1 family helicases have multiple roles in the maintenance of nuclear and mitochondrial DNA in eukaryotes. Saccharomyces cerevisiaePif1 is involved in …
WebNov 26, 2024 · As Pif1 is a 5′–3′ helicase and has critical functions in DNA replication, we designed a substrate to determine whether the BaPif1 molecules bound to both arms of the fork are both active ... medicine hat trailer salesWebDNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Involved in the maintenance of mitochondrial (mtDNA). ... IPR010285 DNA_helicase_pif1-like; IPR027417 P-loop_NTPase; Pfam. View protein in Pfam; PF05970 PIF1 2 hits; SMART. View protein in SMART; SM00382 AAA 1 … medicine hat to saskatchewan borderWebHelicases are molecular engines which translocate along nucleic acids (NA) to unwind double-strands or remodel NA-protein complexes. While they have an essential role in genome structure and expression, the rules dictating their processivity remain medicine hat trailers for saleWebContact. Department of Biology has two locations: 200 College Ave. Medford, MA 02155 Map and Directions Tel: 617-627-2264 Fax: 617-627-3805. 200 Boston Ave. medicine hat trade show 2022WebApr 11, 2024 · We further show that using its translocation activity, Pif1 can push hRPA from a ssDNA loading site into a duplex DNA causing stable disruption of at least 9 bp of duplex DNA. These results highlight the dynamic nature of hRPA enabling it to be readily reorganized even when bound tightly to ssDNA and demonstrate a mechanism by which … nad for back painWebAmong the enzymes thought to process these structures, the Pif1 DNA helicase is. G-rich sequences found at multiple sites throughout all genomes may form secondary structures called G-quadruplexes (G4), which act as roadblocks for molecular motors. Among the enzymes thought to process these structures, the Pif1 DNA helicase is medicine hat tractor partsWebSep 19, 2024 · Pif1 family DNA helicases are conserved from bacteria to humans and have critical and diverse functions in vivo that promote genome integrity. Pif1 family helicases share a 23 amino acid region, called the Pif1 signature … medicine hat transit holiday hours