Clontech pgbkt7

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August undefined, 2024

WebArticle Snippet: AH109 cotransformed with murine pGBKT7-p53/pGADT7-T (supplied by Clontech) was used as positive control, whereas yeast cotransformed with pGADT7/pGBKT7 were used as negative control. β … WebArticle Snippet: AH109 cotransformed with murine pGBKT7-p53/pGADT7-T (supplied by Clontech) was used as positive control, whereas yeast cotransformed with pGADT7/pGBKT7 were used as negative control. β-galactosidase assay was performed …

pGBKT7-53载体质粒图谱、序列、说明书、价格_the - 搜狐

WebApr 21, 2024 · Saccharomyces cerevisiae strain AH109, human fetal brain cDNA library that was constructed on pACT2 fused with the GAL4 activation domain, and control vectors pGADT7, pGADT7-T, pGBKT7-p53, and pGBKT7-Lam were from Clontech. pGBKT7-UL44 was first introduced into strain AH109 and verified that do not activate the report … WebApr 14, 2024 · For expressing the GAL4BD-SMXL5 fusion protein in yeast the open reading frame of the SMXL5 gene was cloned into XmaI/BamHI sites of the pGBKT7 plasmid (Clontech, Palo Alto) resulting in pEW6. recipe for dutch babies in sunset magazine

pGBKT7-Lam载体质粒图谱、序列、说明书、价格_is - 搜狐

http://www.honorgene.com/uploads/old/documents/vector/matchmaker_library_constructionscreening_kits_user_manual.pdf WebApr 11, 2024 · The yeast two-hybrid experiments were performed according to the Yeast Handbook (Clontech, USA). Briefly, full length cDNA of OsUGE1 was amplified and inserted into pGADT7 vector and pGBKT7 vector, respectively. Then fused vectors were co-transformed into yeast strain AH109 and incubated for 3 days at 30 ℃ on medium … WebOct 2, 2024 · Two yeast strains of S. cerevisiae, Y2HGold and Y187 were used in this study (Clontech, California, USA). The Y2HGold yeast ... -SAG2 plasmid was digested by NcoI and PstI restriction enzymes and cloned into the corresponding restriction sites in pGBKT7 yeast vector. The recombinant pGBKT7-SAG2 plasmid was then transformed into … unl study abroad

Yeast two-hybrid vectors - Takara Bio

Web7 rows · pGBKT7 yeast two-hybrid bait vector. pGBKT7 is a yeast two … WebApr 19, 2024 · Culture PCR was performed on at least ten clones for pGADT7-In-T, pGBKT7-Lam and pGBKT7–53 analysis, using 2× Taq PCR StarMix (GenStar, Beijing, China) and the Yu03/04 or Yu03/05 primers, depending on the vector. The resulting PCR … recipe for dutch honeyWebGAL4 DNA-binding domain (DNA-BD) in pGBKT7 vector (Clontech) with the in-fusion cloning method (Clontech). The vector pGBKT7 vector was linearized by primers, infusionA9 and infusionB9. Seven ripG genes, ripG1, ripG2, ripG3, ripG4, ripG5, ripG6, and ripG, were amplified from OE1-1 genome DNA by pairs of primers (Table 2), … unl strive to thrive

"WebDescription from Clontech: "pM is used to generate a fusion of the GAL4 DNA-BD (amino acids 1–147) and a protein of interest in the Matchmaker™ Mammalian Assay Kit 2 (Cat. No. 630305). The hybrid protein is targeted to the cell’s nucleus by the GAL4 nuclear … " - Clontech pgbkt7

Clontech pgbkt7

WebAug 21, 2013 · pGBKT7 Vector Information. Use: pGBKT7 is the DNA-BD Vector included with Clontech's Matchmaker Systems. The MCS of pGBKT7. contains unique restriction sites in frame with the 3' end of the GAL4 DNA-BD for constructing fusion proteins. with a bait protein. The bait protein is also expressed as a fusion to a c-Myc epitope tag. c-Myc … WebOct 18, 2024 · pGBKT7-53 encodes a fusion of the murine p53 protein (a.a. 72–390) and the GAL4 DNA-BD (a.a. 1–147). The murine p53 cDNA (GenBank Accession #K01700) was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. (1993); plasmid modification was performed at …

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Webhi, im currently trying to clone a Nucleoprotein (NP) gene ~1.5kb inside a pGBKT7 vector ~7.3kb. The NP gene contains 2 RE sites, both EcoRI and BamHI. Im using the clontech InFusion cloning strategy.

WebApr 3, 2007 · Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3247-1 Version No. PR742219 I. Introduction continued Optimized vectors facilitate downstream confirmation The Matchmaker System 3 DNA-BD and AD fusion vectors, pGBKT7 and pGADT7, were designed for high-level protein expression and to facilitate confirmationof … WebShow Static Map. Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. Efficient cleavage requires at least two copies of the NaeI recognition sequence. Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends ...

WebAbout our brands: Clontech. Our Clontech brand represents over 30 years of service to the life sciences research community through the development, commercialization, and support of kits and reagents for diverse experimental applications, with emphases in cell … http://faculty.salisbury.edu/~flerickson/Seq%20and%20vectors/pgbkt7%20map.pdf

WebOct 15, 2007 · pGBKT7Prey vector, GAL4 41–147 DNA-BD, LEU2, Km R, c-Myc epitope tag: Clontech pGBKT7-BETF-β-BD fusion in pGBKT7; Km R: This study pGBKT7-PFMPFM-BD fusion in pGBKT7; Km R: This study pGADT7-TSV40 large T-antigen in pGADT7, LEU2, Ap R: Clontech pGBKT7-53Murine p53 in pGBKT7, TRP1, km R: …

WebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using … recipe for dutch meatballsWebOct 19, 2024 · pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. recipe for dutch baby german pancakeWebDec 21, 2015 · The VP37 bait vector (pGBKT7-37) was constructed using the appropriate restriction enzyme (TaKaRa Bio Inc., Otsu, Japan), in order to clone the VP37 genes into pGBKT7 (Clontech Laboratories). The pGBKT7-37 was sequenced prior to transformation to ensure that the bait protein (VP37) encodes in the correct reading frame of the GAL4 … unl sustainability officeWebMap of pGBKT7-53 DNA-BD Control Vector. 55 Figure 19. Map of pGBKT7-Lam DNA-BD Control Vector. 56 Notice to Purchaser Clontech products are to be used for research purposes only. They may not be used for any other purpose, including, but not limited to, … unl sustainability clubWebDec 5, 2008 · pGBKT7 Vector Information. Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3248-5 2 Version No. PR8Y2643. Location of features: • Truncated S. cerevisiae ADH1 . promoter (P. ADH1): 30–736 • GAL4 DNA binding domain (DNA-BD) … recipe for dutch baby with applesWebAug 16, 2024 · The Y2H analysis was carried out using a GAL4-based Y2H system from MatchmakerGold Systems (Clontech, Palo Alto, CA). The full-length complementary DNA (cDNA) of YDA was cut with NotI and SfiI and ligated into pGBKT7 to construct a bait plasmid. ... (Clontech). pGBKT7-Lam and pGADT7-T were used as negative controls. … unl student success hubWebThe p53 fragment was from pGBKT7-p53 used for Y2H. I don’t know whether this fragment is suitable for Y1H. ... According to the Clontech manual for yeast one hybrid kit, it recommends generating ... unl spanish classes